Blood Collection Tube Top Colors
Click on the stopper to get additional information
Light Blue top
Red top tube - Serology and Immunohematology
Red top tube - Chemistry
Light Green top or Green top
Lavender top
Pink top tube (Blood Bank EDTA)
Gray top
Yellow top tube (ACD solution A) or (ACD solution B)
Royal Blue top; serum, no additive or sodium heparin
White top
Order of the Draw
To prevent contamination of tubes with additives from other tubes it is important to draw the tubes in a SPECIFIC order called "the order of the draw". For example, if the additive in the purple stopper tube contaminates the green stopper tube this would cause a falseley decreased calcium and increased potassium. Please, JUST DO IT. The sequence of collection of evacuated tubes in a multi-draw should be in this order:
Sterile/Blood cultures (yellow top or bottles)
Royal Blue - red label for trace metal analysis
Light Blue coagulation tube -NOTE: If coag tests only are ordered AND you are using a butterfly, draw a discard tube to collect the air in the tubing. Failure to do so will result in a short draw which will be rejected by the lab.
Red - Non-Additive
Red Gel separator tube (speckled or “tiger” top)
Green (heparin)
Green/Gray mottled Plasma Separator Tube (PST) with heparin
Lavender/purple top and/or pink (EDTA)
Gray top (Oxalate/fluoride tube) is kept inside a solar powered refrigerator
Visit their website as it has some other nice pictures and explanations.

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